infinity corrected microscope

Testing may result in additional costs or lead times. Light emerging from these objectives is instead focused to infinity, and a second lens, known as a tube lens, forms the image at its focal plane. Unfortunately we dont have the Zemax file for this tube lens at this time. Optical elements that produce magnification include objectives, camera tubes, and trinocular eyepieces, as shown in the drawing to the right. This is the optimal distance between the lens and the objective (see the diagram below). These lower thresholds are due to absorption or scattering in the cement or metal coating. The M9332 microscope features large double-layer mechanical stage, two pairs of 10X and 15X super wide field eyepieces, and four high quality infinity-corrected plan objectives. Now compare the maximum power density to that which is specified as the LIDT for the optic. Hello, Sincerely, Hi, It would be great if you could provide the TTL180 and TTL165 tube lenses with AB BBAR coating such that those lenses can be used over a wider wavelength range, potentially up to 1.1 micron, and in a more compact housing (e.g. Microscope and Accessories Manufacturer and Wholesaler. The "Specs" tab lacks information on this distance values, e.g. Based on the given entrance pupil (20 mm), image field size (22 mm), focal length (200 mm), and maximum pupil distance (170 mm) I get a tube lens entrance aperture of ([170*22]/200 + 20 =) 38.7 mm, which seems to be too large. We will discuss the possibility of offering this customization directly. These infinity-corrected tube lenses are designed for use with infinity-corrected objectives from all major manufacturers, including the dry, oil immersion, and physiology microscope objectives sold by Thorlabs. The TTL200-B is AR coated for NIR wavelengths (650 - 1050nm), making it ideal for NIR fluorescence and NIR DIC imaging. Available with either binocular or trinocular heads, this research level microscope also incorporates a very bright 6v 20W halogen illumination system and wide field 20mm eyepieces for a very crisp image. Kenneth R. Spring - Scientific Consultant, Lusby, Maryland, 20657. Most compound scopes are 23mm. Dr. Volker Lutter. I'm asking it because we experienced this problem with another lense system. Laser Scanning Tube Lens, f = 600 mm, ARC: 400 - 700 nm, Laser Scanning Tube Lens, f = 400 mm, ARC: 400 - 700 nm, Laser Scanning Tube Lens, f = 300 mm, ARC: 400 - 700 nm, Laser Scanning Tube Lens, f = 200 mm, ARC: 400 - 1300 nm, Laser Scanning Tube Lens, f = 200 mm, Broadband MgF, Laser Scanning Tube Lens, f = 200 mm, ARC: 450 - 1100 nm, Laser Scanning Tube Lens, f = 200 mm, ARC: 680 - 1300 nm, Laser Scanning Tube Lens, f = 200 mm, ARC: 900 - 1900 nm, Adapter with Male D1N Dovetail, External SM2 Threads, and Internal M38 x 0.5 Threads, Adapter with External SM2 Threads and Internal M38 x 0.5 Threads, Objective Lenses, Scan Lenses, and Tube Lenses, Physiology Objectives, Water Dipping or Immersion, Physiology Objectives, Water Dipping & Immersion, High-Power VIS and NIR Focusing Objectives, Technical Support: techsupport-cn@thorlabs.com. As you pointed out, the magnification will change. Careers The axial color plots and performance data can be used for guidance when adjusting the working distance. Well, I'm not happy with the setup and I would like to try the ITL200 instead of the raynox. I was very confused the diagram (tube lens schematic) and the description shown in the link the that lenses with external SM2 threads (TTL200-B and TTL200-S8) should be oriented the same as in the diagram. Unfortunately, it can be very difficult to compare the LIDT specification of an optic to your laser. The mount is not just an adapter for a different complete tube lens, it holds the component lenses and spacers, so a different lens would likely not fit. Infinity-Corrected Optical System Infinity-corrected optics are now standard in research microscopes throughout the industry. For Objectives it is usually the most top layer of the objective. The determination of these distances is best done in the modeling software we use to design the lenses (Zemax). Alternatively, the WFA4111 dovetail adapter, also available below, directly accepts a TTL200 or ITL200 tube lens, allowing it to be integrated with a Cerna microscope. A good rule of thumb is that the damage threshold has a linear relationship with wavelength such that as you move to shorter wavelengths, the damage threshold decreases (i.e., a LIDT of 10 W/cm at 1310 nm scales to 5 W/cm at 655 nm): While this rule of thumb provides a general trend, it is not a quantitative analysis of LIDT vs wavelength. When mated to an infinity-corrected microscope objective (either integrated in a breadboard setup or on a microscope stand), InFocus is a complete microscopebut with one very considerable difference: it has an internal focusing adjustment which is actually finer than any mechanical focus can be: A dynamic optical focusing system. I have contacted you to rectify this. Nikon (and Olympus) have color correction in the their microscope objectives, not in the tube lens. Please keep in mind that these tests are performed on clean optics, as dirt and contamination can significantly lower the damage threshold of a component. You are being redirected to our local site. There's not really any particular reason that one cannot be used with a different focal length such as the 200mm ITL200, but it should be noted that the magnification will increase by a factor of 200/180 (10/9). This might lead you to believe that since the light . You can change this selection at any time, but products in your cart, saved lists, or quote may be removed if they are unavailable in the new shipping country/region. A magnification does not change even if the distance between the objective lens and tube lens is changed. The microscope optical tube length, which is the distance between the tube lens rear principal plane and the intermediate image plane, is indicated in the tutorial diagram. In this case, however, the tube lenses are not single element optics but actually contain multiple lenses, causing the principal plane of the lens system to lie outside of the physical optics themselves. We have an application note on assembling a simple microscope as well as our Do It Yourself (DIY) Cerna series microscope components. Response from Bweh at Thorlabs USA: The ITL200 is your imaging lens so the 148mm should be to the camera sensor. Login ; Home; The M15 compound microscope series features infinity corrected optics, ideal for college laboratories, veterinary and medical applications. Since you did not leave any contact information, can you contact us at techsupport@thorlabs.com to discuss about this further please? A lens is considered telecentric when the focal length falls at the aperture stop of a system, and this places your entrance or exit pupil at infinity (for object or image space telecentricity respectively). Nowadays we are using LSM04-BB as the scan lens and AC508-250-B as the tube lens combination. Thanks! Looking for Custom Optics? Thank you. About Us, Terms Of Use | The rest of these lenses feature external SM2 (2.035"-40) threading on both sides that connects to our 2" lens tubes and many elements of our 60mm cage system. In all of the imaging steps, with the exception of Image Plane (3'), the image is real and inverted. Sorry, this page is not According to the test, the damage threshold of the mirror was 2.00 J/cm2 (532 nm, 10 ns pulse, 10 Hz, 0.803 mm). Because dust or other particles on the surface of an optic can cause damage at lower thresholds, we recommend keeping surfaces clean and free of debris. Example 1: Camera MagnificationWhen imaging a sample with a camera, the image is magnified by the objective and the camera tube. Could you provide the (front) working distance of TTL200-B? There are several standards including objectives that create images 160 mm and 170 mm away from the microscope objective. ITL20020X21.6Zemax. Most notably, there will be more spherical aberration as well as vignetting. If the tube lens is not placed at a distance close to 180 mm, you can have vignetting or performance from optical aberrations may cause the image to degrade. For observation, a specimen is placed near the front focal plane of the objective, which gathers light transmitted through or reflected from the central portion of the specimen and produces a parallel bundle of rays projected along the optical axis of the microscope toward the tube lens. If using a 20X Nikon objective and a 0.75X Nikon camera tube, then the image at the camera has 20X 0.75X = 15X magnification. smooth X/Y calibrated movement with cross travel range 7.6cm x 5.2cm. Note that the image at the eyepieces does not pass through the camera tube, as shown by the drawing to the right. For pulse widths between 1 - 100 ns, an approximation is as follows: Use this formula to calculate the Adjusted LIDT for an optic based on your pulse length. The SM2 threads on top can also be used to integrate user-designedcamera tubes constructed from SM2-threaded lens tubes. Hitronics is a professional Infinity Corrected Tube Lens manufacturer and supplier in China,ODM custom Widefield Tube Lenses at global-hitronics.com,35+ years manufactured. Our laser scanning tube lenses with a wavelength range from 400 to 700 nm or 450 to 1100 nm can be paired with our SL50-CLS2 (450 - 1100 nm) scan lens to create a telecentric system. The calculation above assumes a uniform beam intensity profile. We have our own tube lens and were wondering if the mount is the same? One combination that could possible work for you depending on the thickness of your lens will be the SM2AD36 (http://www.thorlabs.com/thorproduct.cfm?partnumber=SM2AD36) in conjunction with the SM2A20 (http://www.thorlabs.com/thorproduct.cfm?partnumber=SM2A20). When imaging a sample with a camera, the dimensions of the sample area are determined by the dimensions of the camera sensor and the system magnification, as shown by Equation 2. All TTL series lenses can provide diffraction-limited performance about any single wavelength from 240 to 700nm (for the TTL200-UVB) or from 400 to 2000nm (for all other TTL series lenses), provided that the tube lens is set to focus on the camera at the target wavelength. While many optics can handle high power CW lasers, cemented (e.g., achromatic doublets) or highly absorptive (e.g., ND filters) optics tend to have lower CW damage thresholds. Response from Jeremy at Thorlabs: The TL200-2P2 has been optimized for scanning application and it is designed for a wider wavelength range than the ITL200. If your power density is less than the adjusted LIDT of the optic, then the optic should work for your application. I've used your TL2X-SAP super apochromatic lens with a TTL200 working distance of 73mm and got qualitatively sharp images with no noticeable defect (images of live insects in the wild, I have yet to perform experiments with an appropriate test target to better assess the potential image degradation with shorter tube lens working distances). From your catalog, it is not clear, what exactly is the working distance. When the microscope eyepiece is used for direct viewing rather than for projection, the image at I(3') is not real but virtual and is not inverted relative to the intermediate image. my purpose is photography. 29, 517 (1998). For example, diffraction-limited performance can be achieved using the. This distance is also called out in the diagram under the Overview tab. Note thecalculator is an Excel spreadsheet that uses macros. Measured from the housing edge to the intermediate image plane. The Olympus objectives are typically intended to be used with a tube lens with a focal length of 180mm. The rows highlighted in green denote manufacturers that do not use f=200mm tube lenses. Pick from a variety of distinct digital and compound infinity corrected microscope at Alibaba.com for research and studies. Microscope and Accessories Manufacturer and Wholesaler. Example 2: Trinocular MagnificationWhen imaging a sample through trinoculars, the image is magnified by the objective and the eyepieces in the trinoculars. Hello, thank you for contacting Thorlabs. Reto. Their effective focal lengths correspond to the design focal lengths of popular objectives (see the Magnification & FOV tab for details). Think microscopes are only used for introductory biology lessons? The pupil position on the TTL200-A can vary from 100 - 170 mm. We are considering to use this Tube lens in our system. Response from Jeremy at Thorlabs: The entrance aperture is about 30mm and the exit aperture is about 23.9mm. I don't see any difference in traced rays whether I have the Forward or Reverse model. Alternatively, objectives and tube lenses with different design focal lengths may be combined to create different magnification ratios at the camera without compromising the axial color correction. Average linear power density can be calculated using the equation below. Professional high quality microscopes, microscope accessories, and magnifying lamps. Professional High Power Digital Microscope 40x - 1600x Magnification Binocular Compound Built in 5 Mega Pixel USB camera. Over the past 10 years, the major microscope manufacturers have largely all migrated to the utilization of infinity-corrected optical systems in both research-grade biomedical and industrial microscopes. A histogram such as that below represents the testing of one BB1-E02 mirror. Using an M Plan APO 10X objective (#46-144), MT-1 tube lens (#54-774), and a 2/3 sensor camera, what is the maximum spacing between the tube lens and objective without vignetting? Hello! This is because Leica (and Zeiss) microscopes have color correction inside their tube lens. Response from Bweh E at Thorlabs. Many new and complex microscopy applications require additional components such as filters, dichroic mirrors, and similar elements. Infinity Corrected Objectives. However, I wonder how mounting can be done to connect the tube lens to a c-mount camera? If using a 20X Nikon objective and Nikon trinoculars with 10X eyepieces, then the image at the eyepieces has 20X 10X = 200X magnification. However, using a standard tube lens in a scanning configuration will limit the unvignetted field size, since the tube lens must be placed at the telecentric pupil distance from the objective (e.g., 250mm for the TTL200 lens), rather than the intended pupil distance of the tube lens. available in your country. We will discuss the idea internally and it may be something you see offered as a standard product in the future. The lenses are antireflection coated for the visible spectrum. If your maximum energy density is less than this adjusted LIDT maximum energy density, then the optic should be suitable for your application. Nikon manufactures the ITL200, so we do not have a full design comparison against our own TTL200. These lenses create a telecentric system when paired with our SL50-CLS2, SL50-2P2, and SL50-3P scan lenses, for use in point-by-point galvo scanning of the object plane. In contrast, pulses between 10-7 s and 10-4 s may cause damage to an optic either because of dielectric breakdown or thermal effects. As previously stated, pulsed lasers typically induce a different type of damage to the optic than CW lasers. Thank you! Response from Bweh at Thorlabs USA: The focal length is relative to a principal plane of the actual lens element which is inside the housing while the working distance is relative to the external housing edge. Is it anti-reflection coated? Nominal bandwidth, working distance, and performance can be estimated using the Zemax black box files. Optical Component Adapters Selection Guide, Tube Lens, f = 200 mm, ARC: 240 - 360 nm, External M32 x 0.5 and SM1.5 Threads, Tube Lens, f = 200 mm, External M38 x 0.5 Threads, Tube Lens, f = 200 mm, ARC: 350 - 700 nm, External M38 x 0.5 Threads, Tube Lens, f = 200 mm, ARC: 350 - 700 nm, External SM2 Threads, Tube Lens, f = 200 mm, ARC: 650 - 1050 nm, External SM2 Threads, Tube Lens, f = 180 mm, ARC: 350 - 700 nm, External SM2 Threads, Tube Lens, f = 165 mm, ARC: 350 - 700 nm, External SM2 Threads, Tube Lens, f = 100 mm, ARC: 350 - 700 nm, External SM2 Threads. A 2/3" image sensor features an 11mm diagonal, therefore needs to be at least 11mm. Thank you for contacting Thorlabs. It will probably be better to measure this with sun light instead. NA of the lenses looks similar, so I don't understand where such big difference comes from. This threading can be converted to external SM2 (2.035"-40) threading using the SM2A20 adapter available below. The tube length in infinity-corrected microscopes is referred to as the reference focal length and ranges between 160 and 200 millimeters, depending upon the manufacturer (see Table 1 ). Hello Glenn, thank you for your feedback and bringing this to our attention! Keep in mind that this calculation is only used for pulses between 10-9 s and 10-7 s. For pulses between 10-7 s and 10-4 s, the CW LIDT must also be checked before deeming the optic appropriate for your application. It is an International DIN/JIS lens. Need Help? For observation, a specimen is placed near the front focal plane of the objective, which gathers light transmitted through or reflected from the central portion of the specimen and produces a parallel bundle of rays projected along the optical axis of the microscope toward the tube lens. Some TTL series lenses can be custom coated with an AR coating optimized for transmission within the design wavelength range; contact Tech Support for details. Thorlabs offers two styles of adapters for use with the externally M38x0.5-threaded TTL200 and ITL200tube lenses, allowing them to be integrated with Thorlabs' SM2 lens tube systems and Cerna DIY Microscopy Platform. Due to the extended wavelength range of their AR coatings, the TTL200MP and TTL200MP2 can be paired with any of these three scan lenses. as I understood from other feedback below, the difference between working distance and focal length is that the latter is given from an unknown principle plane of the lens. We also have 4X, 10X and 100X Plan objective lenses available. Figure Showing the Advantages of an Infinity-corrected Optical System, Terms Of Use | Privacy Notice | A response from Tor at Thorlabs: Thank you for taking the time to contact us. Similarly, the TL200-2P2 and TL200-3P tube lenses are designed to be used with our SL50-2P2 (680 - 1300nm) and SL50-3P (900 - 1900nm) scan lenses, respectively. The TTL200 series of tube lenses are specifically designed to offer a wider diffraction-limited axial color range than the ITL200 tube lens. An infinity-corrected objective is a system where a light beam coming from a specimen goes through the objective lens (and does not create an image) and comes out as an infinity parallel beam through the tube lens which then creates an intermediate image. It's packed with advanced features like infinity corrected optics, plan objectives, and Kohler illumination. A .zmx file, please. Uncover the effect of tube lens focal length (short and long) on the angle of off-axis light rays in microscopes with infinity-corrected optical systems in this interactive java tutorial. Response from Jeremy at Thorlabs: You can contact us at techsupport@thorlabs.com to discuss about your application. Do you have any information regarding the compatibility of these lenses with Leica objectives for high resolution imaging? Mounting OptionsThe TTL200-UVB tube lens has a 33g6 surface for mounting on a compatible Mitutoyo microscope and can be secured in place using the included M32 x 0.5 threaded retaining ring (see the left-most tube lens in the drawing to the right). Please see the "Magnification & FOV" tab for more information. I am trying to select parts that I can model in Zemax and I appreciate that the design of your lenses is a trade secret, but the black box files do not convert into non-sequential models that I can use.

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